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Dalam makalah ini penulis akan membahas masalah mengenai "Northern Blotting dan Western Blotting" karena sebagai seorang mahasiswa saintist maka kita perlu mengetahui hal ini. Northern Blot - Hiroki Kuroda. Sometimes useful to check amount and size of RNA. (Gel for Northern Blot) -Mix 1 g of agarose, 5 ml of 20 x MOPS, and 77 ml of water using microwave -Store at The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression Northern blotting consists of the following steps: electrophoresis, transfer, and detection of specific The three above mentioned blotting techniques (Western, Southern, and Northern) are modified in Northern Blotting. Howard Judelson, Nov. Electroblotting or capillary blotting are both acceptable. Electroblotting is reported give better results for high molecular weight species but is more difficult Northern blotting by mujahid hussain 37081 views. Southern blot power point by carebeareyre 28353 views. Northern blotting. 1. • Developed by James Alwine, David Kemp and George Stark in 1977.• Northern blotting was one of the first techniques used for studying miRNAs, and remains one of the most valuable as it avoids enzymatic manipulation of miRNA transcripts. The Northern blot is used to detect the presence of a particular mRNA in a sample. The term "Northern" has no scientific significance just a misnomer. Principle: The key to this method is Function of Southern Blot. • determine the molecular weight of a restriction fragment and to measure relative amounts in different samples. • detect the presence of a particular bit of DNA in a sample Northern blotting is one of several effective methods that can be used to analyze the steady-state Northern blotting was the first procedure developed for analyzing the molecular size and abundance Northern blotting is the main method for examining the expression of genes through measurement of an mRNA. Tissue specificity and the factors which regulate expression can both be determined by

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